Cysteine- and glycine-rich protein 1 predicts prognosis and therapy response in patients with acute myeloid leukemia

Acute myeloid leukemia (AML) is a heterogeneous disease with a poor prognosis. The current risk stratification system is essential but remains insufficient to select the best schedules. Cysteine-rich protein 1 (CSRP1) is a member of the CSRP family and associated with poor clinicopathological features in many tumors. This study aimed to explore the clinical significance and molecular mechanisms of cysteine- and glycine-rich protein 1 (CSRP1) in AML. RT-qPCR was used to detect the relative expression of CSRP1 in our clinical cohort. Functional enrichment analysis of CSRP1-related differentially expressed genes was carried out by GO/KEGG enrichment analysis, immune cell infiltration analysis, and protein–protein interaction (PPI) network. The OncoPredict algorithm was implemented to explore correlations between CSRP1 and drug resistance. CSRP1 was highly expressed in AML compared with normal samples. High CSRP1 expression was an independent poor prognostic factor. Functional enrichment analysis showed neutrophil activation and apoptosis were associated with CSRP1. In the PPI network, 19 genes were present in the most significant module, and 9 of them were correlated with AML prognosis. The high CSRP1 patients showed higher sensitivity to 5-fluorouracil, gemcitabine, rapamycin, cisplatin and lower sensitivity to fludarabine. CSRP1 may serve as a potential prognostic marker and a therapeutic target for AML in the future. Supplementary Information The online version contains supplementary material available at 10.1007/s10238-023-01269-w.


Introduction
Acute myeloid leukemia (AML) is a heterogeneous and highly aggressive hematologic malignancy [1].It is the most prevalent form of acute leukemia in adults, with an annual incidence of 4.3/100,000 and an increasing incidence risk with older age [2].Existing standard treatments include intensive chemotherapy and allogeneic hematopoietic stem cell transplantation (HSCT).Since 2017, the U.S. Food and Drug Administration (FDA) has approved eight new drugs for the treatment of AML, including the FLT3 inhibitors midostaurin and gicitinib, the IDH inhibitors efusitinib and enalcitinib, the anti-CD33 monoclonal antibody gemtuzumab ozogamicin, CPX351, the hedgehog pathway inhibitor glasdegib, and BCL-2 inhibitor venetoclax.Targeted therapies are more effective and less toxic than the conventional chemotherapy [3].Despite significant advances in these new therapies, primary and secondary resistance remains a major obstacle in treating AML.Therefore, preevaluation of the likelihood of resistance is vital for treatment options [4,5].Allogeneic HSCT has become an effective tool for AML cure by preventing AML relapse through high-dose chemotherapy and graft-versus-leukemia effects.However, how to screen the population for transplantation is still a challenge in AML treatment.According to the current cytogenetic risk stratification, patients are classified into three risk groups (favorable group, intermediate group, and adverse group).However, patients classified as favorable or intermediate group still have high relapse and drug resistance rates, suggesting that the current risk stratification system is still inadequate.Therefore, the creation of novel and trustworthy prognostic biomarkers for AML is urgently required.
Cysteine-rich protein 1 (CSRP1) is a member of the CSRP family.This gene family contains a group of LIM domain proteins, which are proposed to be involved in regulatory processes essential for development and cellular differentiation.CSRP1 is located on human chromosome 1q32.1 [6].The LIM protein, CRP1, is a general marker for smooth muscle lineages [7].CRP1 localizes to the nucleus and the cytoplasm with different functions depending on its location.When CRP1 is in the nucleus, it regulates interactions between transcription factors and promotes the upregulation of smooth muscle-specific genes [8].When CRP1 is in the cytoplasm, it localizes to the adhesion patch and the actin cytoskeleton to regulate actin filament bundles [9].
Only a few reports have been made about CSRP1's connection to cancer thus far.CSRP1 is associated with poor clinicopathological features in adrenocortical carcinoma (ACC) [10].Hepatocellular carcinoma (HCC) causes abnormal methylation to inactivate CSRP1, which may be a key biomarker for cancer [11].CSRP1 was used to forecast when benign prostatic hyperplasia will turn into prostate cancer and may have an effect on disease-free survival [12].In addition, CSRP1 is also associated with colorectal cancer [13] and breast cancer [14].
There has been no research to investigate the expression profile and function of CSRP1 in AML.In this study, we examined the database and our cohort to investigate the expression of CSRP1 in AML and its prognostic significance.We also studied the differentially expressed genes related to CSRP1 expression and explored their potential roles in AML through GO and KEGG enrichment, immune infiltration, protein interaction analysis, and drug sensitivity analysis.

Patients and treatment regimen
Bone marrow samples from 224 newly diagnosed AML patients and 23 healthy individuals were obtained at the First Affiliated Hospital of Zhengzhou University between February 2017 and July 2020.We named our cohort as ZZU cohort.The inclusion criteria included: 1) patients were diagnosed as AML in our hospital; 2) patients were ≥ 14 years old; and 3) patients accepted at least one course of chemotherapy in our hospital.The exclusion criteria were patients who didn't treat or treat elsewhere and patients with acute promyelocytic leukemia.Patient clinical information, such as age, gender, risk stratification, FAB classification, gene mutations, and other basic information, was gathered from electronic medical records.All data were collected independently by two researchers who received training to reliably complete all assessments.Details of treatment regimens are reported [15,16].Induction chemotherapy regimens include IA and DA regimens: standard-dose cytarabine (Ara-C) 100-200 mg•m −2 •d −1 × 7 d combined with idarubicin 10-12 mg•m −2 •d −1 × 3d or daunorubicin 60 mg•m −2 •d −1 × 3d.The treatment plan after remission is high-dose Ara-C (2-3 g/m 2 , once every 12 h, 3d).Those who have not undergone HSCT share four courses; those who receive HSCT share two courses, followed by HSCT.For elderly patients over 60 years old or patients who cannot tolerate intensive chemotherapy, they received chemotherapy with demethylating drugs ± CAG ± venetoclax regimen until progression.In total, 38 subjects received an allogeneic HSCT.Subjects were followed up until death, loss to followup or December 2022.The follow-up was conducted through in-clinic follow-up and telephone calls.Patients were classified into low CSRP1 group and high CSRP1 group according to the expression of CSRP1 at diagnosis.The clinical significance of CSRP1 in the AML cohort was analyzed retrospectively.Complete remission, relapse, risk stratification, overall survival (OS), and relapse-free survival (RFS) were defined as described according to the 2017 ELN recommendations from an international expert panel [17].The study was approved by the Ethics Committee of the First Affiliated Hospital of Zhengzhou University, and written informed consent was exempted by the Ethics Committee of the First Affiliated Hospital of Zhengzhou University.

RNA extraction, cDNA synthesis and RT-qPCR
Bone marrow mononuclear cells were obtained via density gradient centrifugation.Total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA).The cDNA was synthesized using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) (18).CSRP1 transcript levels were detected by the Taqman method using real-time quantitative polymerase chain reaction (RT-qPCR) as previously described [18].Serial dilutions of plasmids expressing CSRP1 and ABL1 were amplified to construct standard quantification curves [19].The CSRP1 and ABL1 copy numbers were calculated from standard curves.As previously described, the CSRP1 transcript levels were calculated as the ratio of the CSRP1 copy number/ABL1 copy number [20].The primers and probe sequences are shown in Table S1.

Data acquisition
We obtained clinical information and gene expression profiles for AML patients from three independent sources.The first source is Tyner's study, which included 405 AML patients (hereafter referred to as the Beat-AML dataset) [21].
The second source is the TCGA database, which provides RNA-seq expression data and detailed clinical data on 173 AML patients (hereafter referred to as the TCGA-LAML dataset) [22].The third source is the GSE12417 from the GEO database (https:// www.ncbi.nlm.nih.gov/ geo/; hereafter referred to as the GSE12417 dataset) [23].The AML samples' Level 3 HTSeq-FPKM and HTSeq-Count data were downloaded from the TCGA website (https:// portal.gdc.cancer.gov/ repos itory) for further investigation.

Survival analyses
We utilized the pROC package to determine the optimal cutoff value for each gene and divided AML patients into two subgroups: the "high-expression" and "low-expression" groups according to the cutoff value of the gene.Then we performed Kaplan-Meier survival analysis to investigate the prognostic value of gene expression using the survival package.

Differentially expressed gene (DEG) analysis
The DESeq2 R package was used to compare the expression data of low and high expression of CSRP1 (cutoff value of 50%) in AML samples to identify DEGs [24].Heatmap analysis of the top 20 DEGs was performed.

Functional enrichment analysis of DEGs
Functional enrichment analysis was performed on DEGs with |logFC| > 0.5 and padj < 0.05.The ClusteProfiler package [25] in R was used to implement Gene Ontology (GO) functional analysis, which included cellular component (CC), molecular function (MF), and biological process (BP) analysis, as well as Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.

Analysis of immune infiltration using single-sample gene set enrichment analysis (ssGSEA)
CSRP1 immune infiltration analysis was carried out using ssGSEA utilizing the GSVA package [26] in R. As previously stated [27], 24 kinds of infiltrating immune cells were obtained.The Spearman correction was used to examine the relationship between CSRP1 and the enrichment scores of 24 different kinds of immune cells.The Wilcoxon rank-sum test was used to examine the enrichment scores of the groups with high and low CSRP1 expression.

The protein-protein interaction (PPI) network
The Search Tool for the Retrieval of Interacting Genes (STRING) database (http:// string-db.org) was used to predict the DEG PPI network [28].The cutoff criterion was set at an interaction score level of 0.4.The PPI network was mapped with Cytoscape (version 3.6.1)[29], and the most critical modules in the PPI network were identified with MCODE (version 1.5.1)[30].The following were the selection criteria: MCODE scores greater than 5, degree cutoff = 2, node score cutoff = 0.2, Max depth = 100, and k-score = 2.

Generation and prediction of prognostic models
A nomogram was developed using the rms R package (version 6.2-0) to individualize the prediction of overall survival (OS) in AML patients, which comprised key clinical features and calibration plots.The calibration curves were visually assessed by plotting the nomogram-predicted probabilities against the observed rates, with the 45 line representing the best predictive values.All statistical tests were two-tailed, and the statistical significance threshold was set at 0.05.

Chemotherapy response prediction
The oncoPredict package of R was used to forecast medication sensitivity to 5-fluorouracil, gemcitabine, rapamycin, cisplatin, and fludarabine with the goal of enhancing tailored therapy.Accordingly, drug sensitivity values (measured by AUC, the area under the concentration-response curve) were estimated followed by a comparison of the values between the high and low CSRP1 groups.High AUC means low sensitivity.

Statistical investigation
R (3.6.3) was used to perform and show all statistical analyses and figures.Wilcoxon rank-sum test was used to evaluate CSRP1 expression in unpaired samples, while Wilcoxon signed-rank test was utilized in paired samples.Quantitative data are presented as means ± SD (standard deviations) or medians with interquartile ranges.To assess the connection between clinical/cytogenetic features and CSRP1 expression, the Kruskal-Wallis test, Wilcoxon signed-rank test, and logistic regression analysis were performed.The prognostic variables were evaluated using Cox regression analysis and the Kaplan-Meier technique.The cutoff of CSRP1 was calculated by X-tile.There were few missing data on the key variables in our main analysis.Participants who were lost to follow-up were deemed alive after loss to follow-up.Multivariate analyses were conducted, controlling for confounders.

Overexpression of CSRP1 in AML and expression of CSRP1 in pan-cancers
RNA-seq data were obtained in the TCGA and GTEx databases.By comparing the expression of CSRP1 in normal samples in the GTEX database and corresponding tumor samples in the TCGA database, CSRP1 was found to be significantly overexpressed in 7 cancers, including acute myeloid leukemia (LAML), and underexpressed in 18 cancers (Fig. 1A).For the CSRP gene family, CSRP1 was significantly overexpressed in AML, while CSRP3 was significantly reduced in AML compared to healthy controls (Fig. 1B).Furthermore, by comparing the expression of CSRP1 in 23 normal bone marrow (NBM) and 224 newly diagnosed AML patients in the ZZU cohort, we confirmed that CSRP1 was overexpressed in AML (Fig. 1C).

Association between CSRP1 expression and clinical features
The main clinical characteristics of AML in the ZZU cohort are shown in Table 1.A total of 224 AML patients were included in the prognostic analysis.The median follow-up time was 529 days.Up to the follow-up time, 138 patients died.The AML patients were divided into the low CSRP1 group (109 cases) and the high CSRP1 group (115 cases) according to the cut-off CSRP1 expression (225%).The high CSRP1 group had higher BM-blasts and more DNMT3A mutations than the low CSRP1 group (P < 0.05, Table 1 and Fig. 5G).We also compared the expression of CSRP1 in AML patients by the other characteristics.The expression levels of CSRP1 showed no significant difference in patients with different gender, age, WBC, cytogenetic risk group, BM-blasts, and PB-blasts (P > 0.05; Fig. 2A-F).

High CSRP1 impacted the prognosis of AML
Patients with high CSRP1 expression had a significantly worse prognosis than those with low CSRP1 expression (hazard ratio [HR], 2.36 (1.53-3.64);P < 0.001; Fig. 3A) in the TCGA-LAML dataset.The predictive significance of elevated CSRP1 was verified in the Beat-AML dataset (Fig. 3B), the ZZU cohort (Fig. 3C), and the GSE12417 dataset (Fig. 3D-E).The time-dependent ROC curve from the TCGA-LAML dataset demonstrated that CSRP1 was an excellent predictor of AML patient survival.

Prognostic model of CSRP1 in AML
A nomogram was developed based on the Cox regression analyses in the TCGA dataset (Fig. 4C) and the ZZU cohort (Fig. 4D) to better predict AML patients' prognoses.Three independent prognostic variables, age, cytogenetic risk, and CSRP1 expression, were included in the model at a statistical significance level of 0.05.A point scale was utilized to allocate points to these factors based on multivariate Cox analysis.Results from the nomogram calibration curve of OS prediction were consistent with observations of all patients in both the TCGA dataset (Fig. 4E) and the ZZU cohort (Fig. 4F).

Identification of DEGs in AML samples with low and high CSRP1 expression
The gene expression profiles of the high and low CSRP1 groups were analyzed for differences in median mRNA expression.A total of 2758 DEGs from RNA-seq-HTSeq counts were found to be statistically significant between the high and low CSRP1 groups (|log fold change (logFC)|> 0.5, P adj < 0.05).(Fig. 5A).The heat map depicted the top ten up-regulated DEGs and top ten down-regulated DEGs between the high and low CSRP1 groups (Fig. 5B).
The prognostic value of the top ten up-regulated DEGs and top ten down-regulated DEGs was further investigated.Six of the top ten DEGs that were up-regulated (ONECUT2, CES1, CD163, KCNN2, THBS1, and B3GALT1) and six of the top ten DEGs that were down-regulated (MYT1L, ASTN1, DLL3, MYO18B, POU4F1, and PCDH10) also had vital prognostic significance in the TCGA AML dataset (Fig. 6).

Analysis of immune infiltration in AML
CSRP1 expression in the AML microenvironment was associated with the level of immune cell infiltration as measured by ssGSEA.Mainly, CSRP1 was negatively related to T cells and T helper cells and positively associated with macrophages (Fig. S1C), neutrophils (Fig. S1D), NK CD56

PPI enrichment analysis in AML and validation of hub genes
The PPI network was constructed using the String website and the data were generated by Cytoscape (MCODE plug-in).A total of 321 DEGs were imported into the PPI network.Complete module including all genes is presented in Fig. S2A.We obtained 235 nodes and 900 edges.Nineteen genes were present in the most significant module (MCODE score: 7.667; Fig. S2B).The TCGA-LAML dataset was then used for OS analysis on the hub genes.Nine of the 19 hub genes, including CD163, CX3CR1, C5AR1, THSD7A, ADMATS18, IL10, THBS1, ADAMTS15, and LILRB2, were correlated with OS in AML (P < 0.05, Fig. S3).

CSRP1 expression can predict sensitivity to the common chemotherapy agents in AML treatment
We utilized the "oncoPredict" tool to estimate sensitivity to frequently used chemotherapy agents to better correlate the CSRP1 expression with clinical practice.Accordingly, drug sensitivity of patients in high and low CSRP1 groups to multiple chemotherapy agents including 5-fluorouracil, gemcitabine, rapamycin, cisplatin, and fludarabine was predicted.Based on the findings, the high CSRP1 groups of patients in the TCGA datasets showed higher sensitivity to 5-fluorouracil, gemcitabine, rapamycin, and cisplatin and lower sensitivity to fludarabine (Fig. S4).The relationship of sensitivity to other chemotherapy agents with CSRP1 expression is listed in the supplementary data.

Discussion
In this study, we explored the relationship between CSRP1, the clinicopathological features and prognosis of AML.We found that CSRP1 was highly expressed in adult AML, which was associated with a higher proportion of bone marrow blasts, a higher frequency of DNMT3A mutation and a poor prognosis in AML patients.In addition, we constructed a nomogram to predict OS for AML based on age, cytogenetic risk stratification, and CSRP1 expression levels and explored the possible mechanisms of CSRP1 function.
Through pan-cancer analysis, we found that, unlike other tumor markers that are always highly or lowly expressed in different tumors, CSRP1 shows different expression patterns between different tumors, with CSRP1 highly expressed in 7 cancers and lowly expressed in 18 tumors.It suggests that CSRP1 has a complex mechanism of regulation and can function as either an oncogene or oncogene suppressor in different cancer species or under different circumstances.In addition, the expression of CSRP gene family in AML is inconsistent, with the CSRP1 highly expressed and CSRP3 lowly expressed in AML.We have systematically investigated the role of the CSRP2 gene in AML and found that its low expression is associated with poor prognosis in AML [15].At the same time, the knockdown of CSRP2 promotes proliferation and cycle progression in AML cell lines [15].In contrast, no studies on CSRP1 in AML have been reported.We confirmed the high expression of CSRP1 in AML by comparing 224 adult AML patients with 23 healthy controls, which is consistent with the database results.All of these suggest that CSRP1 may play an essential role in AML.
Next, we investigated the relationship between CSRP1 gene expression, the clinicopathological features, and gene mutations using data from 224 adult AML cases.CSRP1 expression did not correlate with gender, age, risk stratification, or WBC at diagnosis.The analysis of baseline data showed a higher proportion of bone marrow blasts and a higher frequency of DNMT3A mutations in the high CSRP1 group.DNMT3A is one of the most frequently mutated genes in AML [31] and is an independent prognostic factor used for risk stratification [32].It may suggest a higher tumor burden and a higher incidence of adverse prognostic mutations in those with high CSRP1 expression.
We next explored the impact of CSRP1 gene expression levels on overall survival in adult AML patients.We found that high CSRP1 expression was associated with poor OS through multiple adult AML database cohort studies, including TCGA-LAML, Beat-AML, and GEO databases.Moreover, we further validated this result with the ZZU cohort.To further optimize the current stratification system for AML and facilitate clinical application, we first performed a univariate analysis.Initial screening revealed that age > 60 years old, worse cytogenetic risk stratification and high CSRP1 expression were independent poor prognostic factors.The prognostic significance of age and karyotype stratification is well established.To facilitate clinical application, we further developed a nomogram and applied a calibration plot to validate the model.This model performed well for both the TCGA-LAML dataset and the ZZU cohort.This finding is beneficial for further optimizing the stratification system, especially for some patients classified as intermediate risk according to the current stratification, who can now better evaluate transplantation or chemotherapy according to the current guideline recommendation.This stratification system can further score, stratify and guide the treatment selection.
The overexpression of CSRP1 and its associated poor prognostic value in AML suggest that it may play a role in AML.To further explore the mechanism of action of CSRP1, we subjected patients with high and low CSRP1 expression to differential gene expression analysis, followed by GO and KEGG functional enrichment.GO-BP enrichment analysis revealed that CSRP1 was closely associated with neutrophil function, which was confirmed in subsequent microenvironmental correlation analysis.Upregulating neutrophil elastase (NE) promoted the growth of leukemia cells and decreased the proportion of apoptotic cells [33].GO-BP enrichment analysis reveals that CSRP1 is associated with actin binding and Rho GTPs binding.AML with FLT3-ITD mutations is characterized by RAC1-dependent actin cytoskeleton remodeling that substantially contributes to the acquisition of resistance to midostaurin in vitro [34].Yang et al. [35] analyzed the expression patterns and prognostic significance of Rho family GTPases in AML and found that RhoBTB3 was significantly downregulated in AML bone marrow compared to healthy controls and correlated with prognosis of AML.KEGG enrichment analysis revealed that CSRP1 was associated with cell adhesion molecules, rap1 signaling pathway, HIF-1 signaling pathway, JAK-STAT signaling pathway, and apoptosis.Cellular adhesion molecules also impact the poor prognosis of AML and may be used as targets for AML-specific therapies [36].Also, the Rap1 signaling pathway plays a crucial role in cancer [37].HIF signaling has been implicated in myeloid cell survival, and PI3K/Akt is known to induce HIF-1 transcription [38].The signaling pathway JAK/STAT plays a critical role in the development and progression of AML [39].
In the PPI analysis, nine out of the 19 hub genes (CD163, CX3CR1, C5AR1, THSD7A, ADMATS18, IL10, THBS1, ADAMTS15, and LILRB2) were correlated with OS in AML.Notably, CD163 and THBS1 were among the 20 genes screened for the most significant differences based on CSRP1 expression levels.CD163 was a specific marker for macrophages of M2 type and was identified as a potential target for the therapeutic intervention of AML [40,41].The association of CSRP1 with poor prognosis and macrophages in AML may be acting through CD163, but this requires further experimental confirmation.THBS1 is a novel serum prognostic factors of AML [42].CX3CR1 was identified as one of the distinctive features of AML cells for universal MRD monitoring [43].For AML patients who are ineligible for standard treatment with chemotherapy and HSCT and who also experience less severe side effects, Laura Jimbu et al. propose that manipulation of both the co-inhibitory network (with anti-PD-L1 blocking antibodies) and suppressor network (with anti-IL-10 blocking antibodies) is an appealing immunotherapeutic intervention.To increase the therapeutic effectiveness of treating AML illness, the suggested combination of these two immunotherapies provides a novel strategy that can be easily used in the clinic [44].Endothelial cells (ECs)-derived small extracellular vesicles contained a high level of ANGPTL2, which accelerated leukemia progression via binding to the LILRB2 receptor [45].The other genes, including C5AR1, THSD7A, ADAMTS18, and ADAMTS15, have not yet been reported in AML-related studies and also require further investigation.
Finally, we also aimed to investigate whether CSRP1 expression is capable of prognosticating the patients' response to the commonly used chemotherapeutic agents.Our findings revealed that patients with higher CSRP1 expression were more sensitive to certain chemotherapy agents including 5-fluorouracil, gemcitabine, rapamycin, and cisplatin and more resistant to fludarabine.This will help us in the selection of drugs in case of relapse and refractory patients.In summary, CSRP1 was highly expressed in adult AML, and such high-level expression of CSRP1 was related to a poor prognosis in adult AML.CSRP1 may serve as a potential prognostic marker and a therapeutic target for AML in the future.Further verification is expected to be carried out to reveal the clinical significance and biological impacts of CSRP1 in AML.

Fig. 1
Fig.1 AML samples showed a higher expression of CSRP1 compared to normal samples.A Expression levels of CSRP1 in paired samples of normal and tumor patients in different cancers.B Comparisons of gene expressions among different CSRP family for normal and AML

Fig. 2 Fig. 3
Fig.2 Association between CSRP1 expression and clinical features in the ZZU cohort.A-F Comparisons of CSRP1 expression in AML patients based on gender A, age B, cytogenetics risk C, WBC D, dim cells, NK CD56 bright cells, Tem, and iDC, TFH, Th17 cells, Th1 cells, eosinophils, Treg, and aDC (Fig.S1A).Patients with high CSRP1 had higher enrichment scores of aDC, Eosinophils, iDC, macrophages, neutrophils, NK CD56 bright cells, NK CD56 dim cells, Tem, Th1 cells, Treg, and lower enrichment scores of T helper cells.

Fig. 4
Fig.4 Construction of the nomogram model for AML patients.A-B The forest plots showed that age, cytogenetic risk, and CSRP1 expression are independent factors for poor prognosis in the TCGA-LAML dataset A and the ZZU cohort B. C-D Nomogram for predicting the

Fig. 5
Fig.5 GO/KEGG enrichment analysis of DEGs comparing patients with high or low CSRP1 expression in the TCGA-LAML dataset.A Volcano map of the DEGs, including 862 down-regulated genes and 1896 up-regulated genes.B Heat map showing the top ten up-regulated and the top ten down-regulated genes.The samples are shown on the X-axis, while the DEGs are shown on the Y-axis.C-D GO

Fig. 6
Fig.6 Prognostic value of DEGs in TCGA-LAML dataset.A-F Six of the top ten up-regulated DEGs (ONECUT2 A, CES1 B, CD163 C, KCNN2 D, THBS1 E, and B3GALT1 F) showed an adverse effect

Table 1
Association between CSRP1 expression and clinical features in the ZZU cohort